Construct Design

  • Expert sequence analysis of Protein-of-Interest (POI) to increase success rate
  • Optional choice of fusion protein
  • Select any affinity tags or protease sites
  • Commercially synthesized in vector of choice

Protein Expression

  • E. coli based expression
  • Experience with wide variety of expression strains
  • Small-scale expression validation and optimization
  • Large-scale expression (2-20 L) in various medias
  • Larger scales available through CBB

Protein Purification

  • Custom purification protocols to obtain highly pure and functional proteins
  • Buffer optimization for complexes, oligomers, and stability
  • Scales from ~10 ug to 1000 mg
  • Generation of custom nanobody resins

Quality Control

  • SEC and SDS-PAGE analysis included by default
  • Additional options: DLS, mass photometry, and negative stain TEM
  • Freeze/thaw condition testing

Custom Protein Production Timeline

  • Construct Design

    1 week

  • Order Gene Synthesis

    2-3 weeks

  • Small Scale Expression/Purification

    1 week

  • Large Scale Expression

    1 week

  • Purification

    1 week

  • Quality Control

    <1 week

  • Average Project Completion

    8-10 weeks